Journal:

Article Title: Characterization of CD4 – CD8 – CD3 + T-cell receptor-?? + T cells in murine cytomegalovirus infection

doi: 10.1046/j.1365-2567.2000.00052.x

Figure Lengend Snippet: Appearance of DN TCRαβ T cells in peritoneal cavity, liver and spleen after MCMV infection. C57BL/6 and BALB/c mice were infected intraperitoneally with 1 × 105 and 4 × 103 PFU, respectively. The non-adherent PEC, liver and spleen lymphocytes were harvested on days 0, 3, 5, 7, 10 and 15 after infection and the DN TCRαβ+ T cells were determined by FACS analysis as described in the Materials and Methods. ▪ and □ represent the DN TCRαβ+ T cells of C57BL/6 and BALB/c mice, respectively, and ▴ and ▵ are for the combined populations of CD4+ and CD8+ TCRαβ+ T cells of C57BL/6 and BALB/c mice, respectively. The figure represents one of the three independent experiments with similar results.

Article Snippet: After washing with fluorescence-activated cell sorter (FACS) Hanks' washing buffer [Hank's solution ‘Nissui’ (without phenol red) 1%, Na 2 HCO 3 0·03%, NaN 3 0·1%, heat-inactivated FCS 5%, and gentamycin 0·1 mg/ml in double distilled H 2 O], specific stainings are performed as described below.

Techniques: Infection